Coding

Part:BBa_K1683005:Design

Designed by: Matthew W Mortensen   Group: iGEM15_WLC-Milwaukee   (2015-09-17)

E. coli tolC signaling sequence + Pseudomonas aeruginosa oprM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 102
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The main consideration when designing the signaling sequence and Pseudomonas aeruginosa PA01 oprM gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.



Source

The tolC signaling sequence and Pseudomonas aeruginosa PA01 oprM sequence were synthesized from DNA sequences found in genomic databases.

References